real time pcr system (Roche)
Roche manufactures this product 
99
Structured Review
Roche
real time pcr system
Real Time Pcr System, supplied by Roche, used in various techniques. Bioz Stars score: 99/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/real+time+pcr+system/LightCycler+480+System/pmc12996981-51-18-21
Average 99 stars, based on 6 article reviews
Real Time Pcr System, supplied by Roche, used in various techniques. Bioz Stars score: 99/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/real+time+pcr+system/LightCycler+480+System/pmc12996981-51-18-21
Average 99 stars, based on 6 article reviews
real time pcr system - by Bioz Stars,
2026-09
99/100 stars
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RNA Extraction:Article Title: A novel and evolutionarily distinct flavoprotein monooxygenase drives skatole degradation in Rhodococcus . Article Snippet: .. Total RNA was extracted from bacterial cultures using a Vazyme RNA extraction kit and reverse-transcri bed into cDNA using the Hiscript III Reverse Transcriptase kit (Vazyme, China). qPCR reactions were performed using ChamQ Universal SYBR qPCR Master Mix (Vazyme, China) on a Reverse Transcription:Article Title: A novel and evolutionarily distinct flavoprotein monooxygenase drives skatole degradation in Rhodococcus . Article Snippet: .. Total RNA was extracted from bacterial cultures using a Vazyme RNA extraction kit and reverse-transcri bed into cDNA using the Hiscript III Reverse Transcriptase kit (Vazyme, China). qPCR reactions were performed using ChamQ Universal SYBR qPCR Master Mix (Vazyme, China) on a Article Title: An RNA-centric interactomics screen identifies novel proviral and antiviral genome binding interactors for tick-borne encephalitis virus Article Snippet: .. RNA was extracted from cell lysate derived from ChIRP-MS samples using TRizol (Invitrogen, Waltham, USA) and eluted in 30 μl of RNAse-free H 2 O (Qiagen, Venlo, Netherlands) For quantifying TBEV RNA, quantitative reverse transcriptase PCR (RT-qPCR) was performed on 2 μl of extracted RNA using primers specific for the envelope gene (E) of TBEV Hypr (5′ TGT TTC CAT GGC AGA GCC AG and 5′ TCC TTG AGC TTG ACA AGA CAG) and probe (5′ 6-carboxyfluorescein [FAM]- T GGA ACA CCT TCC AAC GGC TTG GCA-minor groove binder nonfluorescent quencher [MGB-NFQ]) (Eurobio, Les Ulis, France) and the qScript XLT 1-Step RT-qPCR ToughMix (Quantabio, Beverly, USA) using the LightCycler 96 Real-time Polymerase Chain Reaction:Article Title: A novel and evolutionarily distinct flavoprotein monooxygenase drives skatole degradation in Rhodococcus . Article Snippet: .. Total RNA was extracted from bacterial cultures using a Vazyme RNA extraction kit and reverse-transcri bed into cDNA using the Hiscript III Reverse Transcriptase kit (Vazyme, China). qPCR reactions were performed using ChamQ Universal SYBR qPCR Master Mix (Vazyme, China) on a Article Title: An RNA-centric interactomics screen identifies novel proviral and antiviral genome binding interactors for tick-borne encephalitis virus Article Snippet: .. RNA was extracted from cell lysate derived from ChIRP-MS samples using TRizol (Invitrogen, Waltham, USA) and eluted in 30 μl of RNAse-free H 2 O (Qiagen, Venlo, Netherlands) For quantifying TBEV RNA, quantitative reverse transcriptase PCR (RT-qPCR) was performed on 2 μl of extracted RNA using primers specific for the envelope gene (E) of TBEV Hypr (5′ TGT TTC CAT GGC AGA GCC AG and 5′ TCC TTG AGC TTG ACA AGA CAG) and probe (5′ 6-carboxyfluorescein [FAM]- T GGA ACA CCT TCC AAC GGC TTG GCA-minor groove binder nonfluorescent quencher [MGB-NFQ]) (Eurobio, Les Ulis, France) and the qScript XLT 1-Step RT-qPCR ToughMix (Quantabio, Beverly, USA) using the LightCycler 96 Article Title: Nanoparticle-Loaded Injectable Hydrogel Alleviates Titanium Particle-Induced Osteolysis by Disrupting GATA6/DDX3X-Mediated Macrophage Inflammation Article Snippet: PrimeScript RT Master Mix (RR036D, TaKaRa Biotechnology) was used to convert RNA to cDNA. .. Then, quantitative real-time polymerase chain reaction (qRT-PCR) was conducted in a LightCycler 96 Article Title: Systematic discovery of pro- and anti-HIV host factors in primary human CD4+ T cells. Article Snippet: Viral and cellular DNA were extracted from HeLa-based cell lines 24 hours post-infection using the QIAamp Blood Mini Kit (Qiagen). .. Quantitative PCR was performed as previously described105 using LightCycler 480 Probes Master reaction mix (Roche) on a LightCycler 96 Article Title: Untargeted Metabolomic Study of the Anti-HBV Effects of Erythrocentaurin In Vitro and In Vivo Using UPLC-Q-TOF/MS. Article Snippet: Erythrocentaurin is a key bioactive metabolite derived from the intestinal biotransformation of swertiamarin and gentiopicroside, two predominant iridoid glycosides in Swertia herbs that have antihepatitis B virus activity.. HBVTg mice and HepG2.2.15 cells were used as in vivo and in vitro models to detect HBsAg and HBeAg, and also determine HBV DNA levels, by conducting ELISA and qPCR.. We also performed untargeted metabolomics via UPLCQTOF/MS. Article Title: Valorization of xylose mother liquor into salidroside by engineered Kluyveromyces marxianus Article Snippet: Total RNA was extracted using the Spin Column Yeast Total RNA Purification Kit (Sangon Biotech, Shanghai), and first-strand cDNA was synthesized using ToloScript All-in-one RT EasyMix for qPCR (Tolo Biotech, Shanghai) following the manufacturers’ instructions. .. RT-qPCR was performed with 2 × Q3 SYBR qPCR Master Mix (Tolo Biotech, Shanghai) on a LightCycler 96 Article Title: Genetic mapping and identification of a major quantitative trait locus associated with the high fiber strength introgressed from Gossypium barbadense. Article Snippet: Key message The high fiber strength (FS) of a Upland cotton cultivar bred by interspecific cross was found to be associated with a D11 QTL and the identification of a candidate gene, and a genetic variant provides a potential tool for molecular breeding of high FS cotton cultivars.. Abstract Fiber strength (FS) is one of the critical determinants of cotton fiber quality.. Despite many quantitative trait loci (QTLs) associated with FS have been documented, many more are yet to be uncovered. Article Title: Neuroprotective effects of hydrogen sulfide on BDNF, IGF-1, and oxidative stress in the hippocampus of high-fat diet rats Article Snippet: Only high-quality RNA samples were used for cDNA synthesis and qPCR analyses, which was performed with a commercial cDNA synthesis kit (Yekta Tajhiz Azma, Iran). .. Relative gene expression was quantified using the Roche LightCycler® 96 Derivative Assay:Article Title: An RNA-centric interactomics screen identifies novel proviral and antiviral genome binding interactors for tick-borne encephalitis virus Article Snippet: .. RNA was extracted from cell lysate derived from ChIRP-MS samples using TRizol (Invitrogen, Waltham, USA) and eluted in 30 μl of RNAse-free H 2 O (Qiagen, Venlo, Netherlands) For quantifying TBEV RNA, quantitative reverse transcriptase PCR (RT-qPCR) was performed on 2 μl of extracted RNA using primers specific for the envelope gene (E) of TBEV Hypr (5′ TGT TTC CAT GGC AGA GCC AG and 5′ TCC TTG AGC TTG ACA AGA CAG) and probe (5′ 6-carboxyfluorescein [FAM]- T GGA ACA CCT TCC AAC GGC TTG GCA-minor groove binder nonfluorescent quencher [MGB-NFQ]) (Eurobio, Les Ulis, France) and the qScript XLT 1-Step RT-qPCR ToughMix (Quantabio, Beverly, USA) using the LightCycler 96 Polymerase Chain Reaction:Article Title: An RNA-centric interactomics screen identifies novel proviral and antiviral genome binding interactors for tick-borne encephalitis virus Article Snippet: .. RNA was extracted from cell lysate derived from ChIRP-MS samples using TRizol (Invitrogen, Waltham, USA) and eluted in 30 μl of RNAse-free H 2 O (Qiagen, Venlo, Netherlands) For quantifying TBEV RNA, quantitative reverse transcriptase PCR (RT-qPCR) was performed on 2 μl of extracted RNA using primers specific for the envelope gene (E) of TBEV Hypr (5′ TGT TTC CAT GGC AGA GCC AG and 5′ TCC TTG AGC TTG ACA AGA CAG) and probe (5′ 6-carboxyfluorescein [FAM]- T GGA ACA CCT TCC AAC GGC TTG GCA-minor groove binder nonfluorescent quencher [MGB-NFQ]) (Eurobio, Les Ulis, France) and the qScript XLT 1-Step RT-qPCR ToughMix (Quantabio, Beverly, USA) using the LightCycler 96 Article Title: Genetic mapping and identification of a major quantitative trait locus associated with the high fiber strength introgressed from Gossypium barbadense. Article Snippet: Key message The high fiber strength (FS) of a Upland cotton cultivar bred by interspecific cross was found to be associated with a D11 QTL and the identification of a candidate gene, and a genetic variant provides a potential tool for molecular breeding of high FS cotton cultivars.. Abstract Fiber strength (FS) is one of the critical determinants of cotton fiber quality.. Despite many quantitative trait loci (QTLs) associated with FS have been documented, many more are yet to be uncovered. Quantitative RT-PCR:Article Title: An RNA-centric interactomics screen identifies novel proviral and antiviral genome binding interactors for tick-borne encephalitis virus Article Snippet: .. RNA was extracted from cell lysate derived from ChIRP-MS samples using TRizol (Invitrogen, Waltham, USA) and eluted in 30 μl of RNAse-free H 2 O (Qiagen, Venlo, Netherlands) For quantifying TBEV RNA, quantitative reverse transcriptase PCR (RT-qPCR) was performed on 2 μl of extracted RNA using primers specific for the envelope gene (E) of TBEV Hypr (5′ TGT TTC CAT GGC AGA GCC AG and 5′ TCC TTG AGC TTG ACA AGA CAG) and probe (5′ 6-carboxyfluorescein [FAM]- T GGA ACA CCT TCC AAC GGC TTG GCA-minor groove binder nonfluorescent quencher [MGB-NFQ]) (Eurobio, Les Ulis, France) and the qScript XLT 1-Step RT-qPCR ToughMix (Quantabio, Beverly, USA) using the LightCycler 96 Article Title: Valorization of xylose mother liquor into salidroside by engineered Kluyveromyces marxianus Article Snippet: Total RNA was extracted using the Spin Column Yeast Total RNA Purification Kit (Sangon Biotech, Shanghai), and first-strand cDNA was synthesized using ToloScript All-in-one RT EasyMix for qPCR (Tolo Biotech, Shanghai) following the manufacturers’ instructions. .. RT-qPCR was performed with 2 × Q3 SYBR qPCR Master Mix (Tolo Biotech, Shanghai) on a LightCycler 96 Article Title: Genetic mapping and identification of a major quantitative trait locus associated with the high fiber strength introgressed from Gossypium barbadense. Article Snippet: Key message The high fiber strength (FS) of a Upland cotton cultivar bred by interspecific cross was found to be associated with a D11 QTL and the identification of a candidate gene, and a genetic variant provides a potential tool for molecular breeding of high FS cotton cultivars.. Abstract Fiber strength (FS) is one of the critical determinants of cotton fiber quality.. Despite many quantitative trait loci (QTLs) associated with FS have been documented, many more are yet to be uncovered. SYBR Green Assay:Article Title: Nanoparticle-Loaded Injectable Hydrogel Alleviates Titanium Particle-Induced Osteolysis by Disrupting GATA6/DDX3X-Mediated Macrophage Inflammation Article Snippet: PrimeScript RT Master Mix (RR036D, TaKaRa Biotechnology) was used to convert RNA to cDNA. .. Then, quantitative real-time polymerase chain reaction (qRT-PCR) was conducted in a LightCycler 96 Chromatography:Article Title: Untargeted Metabolomic Study of the Anti-HBV Effects of Erythrocentaurin In Vitro and In Vivo Using UPLC-Q-TOF/MS. Article Snippet: Erythrocentaurin is a key bioactive metabolite derived from the intestinal biotransformation of swertiamarin and gentiopicroside, two predominant iridoid glycosides in Swertia herbs that have antihepatitis B virus activity.. HBVTg mice and HepG2.2.15 cells were used as in vivo and in vitro models to detect HBsAg and HBeAg, and also determine HBV DNA levels, by conducting ELISA and qPCR.. We also performed untargeted metabolomics via UPLCQTOF/MS. Mass Spectrometry:Article Title: Untargeted Metabolomic Study of the Anti-HBV Effects of Erythrocentaurin In Vitro and In Vivo Using UPLC-Q-TOF/MS. Article Snippet: Erythrocentaurin is a key bioactive metabolite derived from the intestinal biotransformation of swertiamarin and gentiopicroside, two predominant iridoid glycosides in Swertia herbs that have antihepatitis B virus activity.. HBVTg mice and HepG2.2.15 cells were used as in vivo and in vitro models to detect HBsAg and HBeAg, and also determine HBV DNA levels, by conducting ELISA and qPCR.. We also performed untargeted metabolomics via UPLCQTOF/MS. Imaging:Article Title: Untargeted Metabolomic Study of the Anti-HBV Effects of Erythrocentaurin In Vitro and In Vivo Using UPLC-Q-TOF/MS. Article Snippet: Erythrocentaurin is a key bioactive metabolite derived from the intestinal biotransformation of swertiamarin and gentiopicroside, two predominant iridoid glycosides in Swertia herbs that have antihepatitis B virus activity.. HBVTg mice and HepG2.2.15 cells were used as in vivo and in vitro models to detect HBsAg and HBeAg, and also determine HBV DNA levels, by conducting ELISA and qPCR.. We also performed untargeted metabolomics via UPLCQTOF/MS. Microscopy:Article Title: Untargeted Metabolomic Study of the Anti-HBV Effects of Erythrocentaurin In Vitro and In Vivo Using UPLC-Q-TOF/MS. Article Snippet: Erythrocentaurin is a key bioactive metabolite derived from the intestinal biotransformation of swertiamarin and gentiopicroside, two predominant iridoid glycosides in Swertia herbs that have antihepatitis B virus activity.. HBVTg mice and HepG2.2.15 cells were used as in vivo and in vitro models to detect HBsAg and HBeAg, and also determine HBV DNA levels, by conducting ELISA and qPCR.. We also performed untargeted metabolomics via UPLCQTOF/MS. cDNA Synthesis:Article Title: Genetic mapping and identification of a major quantitative trait locus associated with the high fiber strength introgressed from Gossypium barbadense. Article Snippet: Key message The high fiber strength (FS) of a Upland cotton cultivar bred by interspecific cross was found to be associated with a D11 QTL and the identification of a candidate gene, and a genetic variant provides a potential tool for molecular breeding of high FS cotton cultivars.. Abstract Fiber strength (FS) is one of the critical determinants of cotton fiber quality.. Despite many quantitative trait loci (QTLs) associated with FS have been documented, many more are yet to be uncovered. Gene Expression:Article Title: Neuroprotective effects of hydrogen sulfide on BDNF, IGF-1, and oxidative stress in the hippocampus of high-fat diet rats Article Snippet: Only high-quality RNA samples were used for cDNA synthesis and qPCR analyses, which was performed with a commercial cDNA synthesis kit (Yekta Tajhiz Azma, Iran). .. Relative gene expression was quantified using the Roche LightCycler® 96 |